Storage, Stability, and Shipping
: Store product at –70o
C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
: Kinase Assay, Western Blot
: ~69kDa (A2), ~36kDa (B2), ~41kDa (G1)
: Subunit A2: PRKAA2, AMPK, AMPK2, PRKAA
Subunit B2: PRKAB2, MGC61468
Subunit G1: PRKAG1, AMPKG, MGC8666
: AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis (1). AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKKβ (2).
1. Hardie, G.D. The AMP-activated protein kinase pathway – new players upstream and downstream. J. Cell Sci. 2004;117: 5479–5487.
2. Kahn, B.B. et al. AMP-activated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab; 2005: 1, 15–25.
||Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
||Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.
The AMPK (A2/B2/G1), Active product can be utilized in the following research areas, but not limited to:
Apoptosis/Autophagy, Cancer, Cellular Stress, Metabolic Disorder, Ser/Thr Kinases