Description: Mouse Monoclonal Antibody

Specificity: Recognizes the ASK1 protein

Cited Applications: WB

Cross Reactivity: Western blot of human ASK1

ASK1 from other species may also be detectable

Host / Clone#: Mouse, A1

Immunogen: Human recombinant 649-946 ASK1

Formulation: 10mM PBS, pH 7.2

Purification: Affinity Chromatography

Stability: Store at 4°C (add 0.1% NaN₃) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Overexpression of ASK1 induces apoptotic cell death, and ASK1 is activated in cells treated with tumor necrosis factor-alpha (1). ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation (2).

References: 
1. Ichijo, H. et al: Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 275: 90-94, 1997.

2. Nishitoh, H. et al: ASK1 is essential for JNK/SAPK activation by TRAF2. Molec. Cell 2: 389-395, 1998.

		Sample Data:  Representative western blot (1:1000) with Anti-ASK1 (A1) using 20 ng of GST-tagged human full-length recombinant ASK1.

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