: Recognizes the HSP27 protein
: Western blot HSP27 from human cells
HSP27 from other species may also be detectable
: Synthetic peptide corresponding to amino acids 80-84 of
human HSP27 (protein accession number P04792)
: PBS, pH 7.4, 0.02% NaN3, 50% glycerol
: Affinity chromatography
: Store at 4o
C (add 0.1% NaN3
) for several months, and at -20o
C for longer periods.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
: HSP27 is a member of heat shock proteins (HSPs) which
are synthesized in cells in response to heat shock and
other metabolic stresses and provide a transient state of
thermotolerance. HSP27 plays a major role in the
increased thermal resistance acquired by cells after
exposure to HSP inducers (1). The level of HSP27
phosphorylation is significantly elevated after exposure of
cells to heat shock, sodium arsenite, IL-1 and TNF-α.
MAPKAPK2 and MAPKAPK3 are both activated by these
conditions and can phosphorylate HSP27 on serine
1. Landry, J. et al: Heat shock resistance conferred by
expression of the human HSP27 gene in rodent cells. J Cell
Biol. 1989 Jul;109(1):7-15.
2. Clifton, A D. et al: A comparison of the substrate specificity
of MAPKAP kinase-2 and MAPKAP kinase-3 and their
activation by cytokines and cellular stress. FEBS Lett. 1996
||Sample Data: Representative Western blot with Anti-HSP27 (1:1000) using 3 μg,
10 μg and 30 μg of HeLa cell lysate.
The Anti-HSP27 product can be utilized in the following research areas, but not limited to:
Cancer, Cellular Stress, Inflammation