Description: Recombinant human ITK (352-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Kinase Assay, Western Blot

Molecular Weight: ~53 kDa

Gene Aliases: EMT; LYK; PSCTK2; MGC126257; MGC126258

Scientific Background: ITK is a member of the TEC family of non-receptor tyrosine kinases. ITK is expressed in T-cells and is important for T-cell development and activation through the antigen receptor. ITK require prior activation of Lck, Zap-70 and PI3-kinase for efficient activation and shares major substrates with both Lck and Zap-70 (1). ITK knockout mice show multiple effects on T cell development, cytokine production and T-helper cell differentiation. T cells that lack or express mutant versions of ITK show impaired TCR-induced actin polymerization, cell polarization and regulation of the signaling events involved in cytoskeletal reorganization (2).

References: 
1. August, A. et al: The Tec family of tyrosine kinases in T cells, amplifiers of T cell receptor signals. Int J Biochem Cell Biol. 2002 Oct;34(10):1184-9.

2. Finkelstein, L D. et al: Tec kinases: shaping T-cell activation through actin. Trends Cell Biol. 2004 Aug;14(8):443-51.

		Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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