Description: Rabbit Polyclonal Antibody

Specificity: Recognizes the CDC25A protein

Cited Applications: WB

Cross Reactivity: Western blot human and mouse CDC25A

CDC25A from other species may also be detectable

Host : Rabbit

Immunogen: Synthetic peptide corresponding to amino acids 74-78 of human CDC25A

Formulation: PBS, pH 7.4, 0.02% NaN3, 50% glycerol

Purification: Affinity chromatography

Stability: Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: CDC25A is a member of the CDC25 family of phosphatases and is required for cell cycle progression from G1 to S phase. CDC25A activates the cyclin-dependent kinases by removing two inhibitory phosphate groups on the CDKs. CDC25A is a potential oncogene since it promotes cell division. CDC25A is over-expressed in a number of cancers and functions as an androgen receptor (AR) co-regulator suppressing the AR transcriptional activity. Human cells respond to ultraviolet light or ionizing radiation by rapid, ubiquitin- and proteasome-dependent protein degradation of CDC25A (1).

References: 
1. Falck, J. et al: The ATM-Chk2-Cdc25A checkpoint pathway guards against radioresistant DNA synthesis. Nature 410842-847, 2001.

		Sample Data:  Representative Western blot with Anti-CDC25A (1:1000) using 3 μg, 10 μg and 30 μg of Hela cell lysate.

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