Description: Rabbit Polyclonal Antibody

Specificity: Recognizes the ELK1 protein

Cited Applications: WB

Cross Reactivity: Western blot human ELK1

ELK1 from other species may also be detectable

Host: Rabbit

Immunogen: Synthetic peptide corresponding to amino acids 387-391 of human ELK1

Formulation: PBS, pH7.4, 0.02%NaN3, 50% glycerol

Purification: Affinity Chromatography

Stability: Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: ELK1 is a member of the ETS oncogene family of transcription factors (1). ELK1 protein interacts with promoter of the c-fos proto-oncogene and has been shown to be involved in the Ras-Raf-MAPK signaling cascade (1). Immature phosphorylation of transcription factor ELK-1 is implicated in premature aging syndrome and insulin resistance. Expression studies have revealed that elevated expression of ELK1-like protein in human esophageal can result in squamous cell carcinoma (2).

References: 
1.Chen, A.G. et al: Overexpression of Ets-like protein 1 in human esophageal squamous cell carcinoma. World J Gastroenterol. 2006, 28;12(48):7859-63.

2.Knebel, B. et al: Reduced phosphorylation of transcription factor Elk-1 in cultured fibroblasts of a patient with premature aging syndrome and insulin resistance. Exp Clin Endocrinol Diabetes. 2005;113(2):94-101.

		Sample Data:  Representative Western blot with Anti-ELK1 (1:1000) using 15ng of GST-tagged human recombinant ELK1.

		Sample Data:  Representative Western blot with Anti-ELK1 (1:1000) using 20μg of HeLa, 293, Jurkat and THP1cell lysates.

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