: Recognizes the PKAcα, PKAcβ and PKAcγ protein
: Western blot human PKAcα, PKAcβ and PKAcγ
PKAcα, PKAcβ and PKAcγ from other species may also be detectable
: Protein Code: P17612
: TBS pH 7.2, 50% glycerol
: Affinity purified using Protein G
: Store at 4°C (add 0.1% NaN3
) for several months, and at -20°C for longer periods.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
: The second messenger cyclic AMP (cAMP) activates cAMP-dependent protein kinase (PKA or cAPK) in mammalian cells and controls many cellular mechanismssuch as gene transcription, ion transport and protein phosphorylation (1). Inactive PKA is a heterotetramer composed of a regulatory subunit (R) dimer and a catalytic subunit (C) dimer, and in this inactive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits. Three C subunit isoforms (-cα, -cβand -cγ) and two families of regulatory subunits (RI and RII) with distinct cAMP binding properties have been identified. Upon binding of cAMP to the R subunits, the autoinhibitory contact is eased and active monomeric C subunits are released.
1. Montminy M: Transcriptional regulation by cyclic AMP. Annu Rev Biochem, 1997;66:807-22.
||Sample Western Blot:
Representative western blot with Anti-PKAc (1:250) using 20ng of PKAcα, PKAcβ and PKAcγ recombinant protein.
The Anti-PKAc product can be utilized in the following research areas, but not limited to:
Apoptosis/Autophagy, Cardiovascular Disease, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, PKA/PKC Pathway