Description: Rabbit Polyclonal Antibody

Specificity: Recognizes the PKAcα, PKAcβ and PKAcγ protein

Cited Applications: WB

Cross Reactivity: Western blot human PKAcα, PKAcβ and PKAcγ

PKAcα, PKAcβ and PKAcγ from other species may also be detectable

Host: Rabbit

Immunogen: Protein Code: P17612

Formulation: TBS pH 7.2, 50% glycerol

Purification: Affinity purified using Protein G

Stability: Store at 4°C (add 0.1% NaN₃) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: The second messenger cyclic AMP (cAMP) activates cAMP-dependent protein kinase (PKA or cAPK) in mammalian cells and controls many cellular mechanismssuch as gene transcription, ion transport and protein phosphorylation (1). Inactive PKA is a heterotetramer composed of a regulatory subunit (R) dimer and a catalytic subunit (C) dimer, and in this inactive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits. Three C subunit isoforms (-cα, -cβand -cγ) and two families of regulatory subunits (RI and RII) with distinct cAMP binding properties have been identified. Upon binding of cAMP to the R subunits, the autoinhibitory contact is eased and active monomeric C subunits are released.

References: 
1. Montminy M: Transcriptional regulation by cyclic AMP. Annu Rev Biochem, 1997;66:807-22.

		Sample Western Blot:   Representative western blot with Anti-PKAc (1:250) using 20ng of PKAcα, PKAcβ and PKAcγ recombinant protein.

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