Description: Rabbit Polyclonal Antibody

Specificity: Recognizes the REL A (NFKB/p65) protein

Cited Applications: WB

Cross Reactivity: Western blot human and mouse REL A

REL A from other species may also be detectable

Host : Rabbit

Immunogen: Synthetic peptide corresponding to amino acids 527-531 of human REL A

Formulation: PBS, pH7.4, 0.02%NaN3, 50% glycerol

Purification: Affinity chromatography

Stability: Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses in unstimulated cells, NF-κB is sequestered in the cytoplasm by its inhibitory proteins, the IκBs (1). NF-κB-activating agents can induce the phosphorylation of IκB’s, targeting them for rapid degradation through an ubiquitin-proteasome pathway, releasing NF-κB to enter the nucleus, where it regulates gene expression.

References: 
1. Baeuerle, P. et al: Function and activation of NF-kappa B in the immune system. Annu Rev Immunol. 1994; 12: 141-79.

		Sample Data:  Representative Western blot with Anti-REL A (1:1000) using 20 ng human recombinant Rel A.

		Sample Data:  Representative Western blot with Anti-REL A (1:1000) using 3 μg, 10 μg, and 30 μg of 3T6 cell lysate.

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