: Recognizes the REL A (NFKB/p65) protein
: Western blot human and mouse REL A
REL A from other species may also be detectable
: Synthetic peptide corresponding to amino acids 527-531 of human REL A
: PBS, pH7.4, 0.02%NaN3, 50% glycerol
: Affinity chromatography
: Store at 4o
C (add 0.1% NaN3
) for several months, and at -20o
C for longer periods.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
: Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses in unstimulated cells, NF-κB is sequestered in the cytoplasm by its inhibitory proteins, the IκBs (1). NF-κB-activating agents can induce the phosphorylation of IκB’s, targeting them for rapid degradation through an ubiquitin-proteasome pathway, releasing NF-κB to enter the nucleus, where it regulates gene expression.
1. Baeuerle, P. et al: Function and activation of NF-kappa B in the immune system. Annu Rev Immunol. 1994; 12: 141-79.
||Sample Data: Representative Western blot with Anti-REL A (1:1000) using 20 ng human recombinant Rel A.
||Sample Data: Representative Western blot with Anti-REL A (1:1000) using 3 μg, 10 μg, and 30 μg of 3T6 cell lysate.
The Anti-Rel A product can be utilized in the following research areas, but not limited to:
AKT/PKB Pathway, Apoptosis/Autophagy, Inflammation, p38 Pathway