Description: Recombinant human PI3K (p65 alpha) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. This protein is an N-terminus fragment (1-571) of full-length PIK3R1 (p85 alpha).

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Western Blot

Molecular Weight: ~67 kDa

Gene Aliases: PIK3R1, GRB1, p85-ALPHA

Scientific Background: PIK3R1 is a Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position which comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kDand it encodes the 85 kD regulatory subunit. PIK3R1 protein associates with activated growth factor receptors. p85-alpha modulates the interaction between PI3 kinase and platelet-derived growth factor receptor (1). PI3K plays an essential role in the development and induction of mast cells in normal and pathogenic immune responses (2).Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.

References: 
1. Skolnik, E. Y. et.al: Cloning of PI3-kinase associated p85 utilizing a novel method for expression/cloning of target proteins for receptor tyrosine kinases. Cell 65: 83-90, 1991.

2. Fukao, T. et.al: Selective loss of gastrointestinal mast cells and impaired immunity in PI3K-deficient mice. Nature Immun. 3: 295-304, 2002.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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