Description: Mouse Monoclonal Antibody

Specificity: Anti-AKT1 antibody recognizes the AKT protein.

Cited Applications: WB

Cross Reactivity: Western blot AKT1 from human and mouse cells

AKT from other species may also be detectable.

Host / Isotype / Clone#: Mouse, Monoclonal, 9A4

Immunogen: Recombinant full length active AKT1 protein

Formulation: 0.1M TRIS pH 8.0, 0.1M glycine

Purification: Affinity purified using Protein G

Stability: Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: AKT1/PKBα is a serine/threonine kinase that belongs to the AKT family. AKT1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). AKT1 is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias (2).

References: 
1. Coffer, PJ. et al: Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 1998 Oct 1; 335 (Pt 1): 1-13.

2. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.

		Sample Data:  Representative western blot (1:2500) using 10µg, 20µg, and 40µg of HEK-293 cell lysate.

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