: Anti-AKT1 antibody recognizes the AKT protein.
: Western blot AKT1 from human and mouse cells
AKT from other species may also be detectable.
Host / Isotype / Clone#
: Mouse, Monoclonal, 9A4
: Recombinant full length active AKT1 protein
: 0.1M TRIS pH 8.0, 0.1M glycine
: Affinity purified using Protein G
: Store at 4o
C (add 0.1% NaN3
) for several months, and at -20o
C for longer periods.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
: AKT1/PKBα is a serine/threonine kinase that belongs to the AKT family. AKT1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). AKT1 is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias (2).
1. Coffer, PJ. et al: Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 1998 Oct 1; 335 (Pt 1): 1-13.
2. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.
||Sample Data: Representative western blot (1:2500) using 10µg, 20µg, and 40µg of HEK-293 cell lysate.
The Anti-AKT1 product can be utilized in the following research areas, but not limited to:
AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling