Description: Rabbit Polyclonal Antibody

Specificity: Recognizes the GSK3α protein

Cited Applications: WB

Cross Reactivity: Western blot of human GSK3α

GSK3α from other species may also be detectable

Host: Rabbit

Immunogen: Protein Code P49840

Formulation: TBS, 50% glycerol

Purification: Affinity Chromatography

Stability: Store at 4°C (add 0.1% NaN₃) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: GSK3α is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase and transcription factors (e.g., JUN) (1). GSK3α also plays a role in the WNT and PI3K signaling pathways. Alzheimer disease is associated with increased production and aggregation of amyloid-beta-40 and -42 peptides into plaques. GSK3α is required for maximal production of the beta-amyloid-40 and -42 peptides generated from the amyloid precursor protein by presenilin-dependent gamma-secretase cleavage. In vitro, lithium, a GSK3α inhibitor, blocks the production of the beta-amyloid peptides by interfering with the gamma-secretase step (2).

References: 
1. Ali, A. et al: Glycogen synthase kinase-3 : properties, functions, and regulation. Chem. Rev. 101: 2527-2540, 2001.

2. Phiel, C J. et al: GSK-3-alpha regulates production of Alzheimer's disease amyloid-beta peptides. Nature 423: 435-439, 2003.

		Sample Data:  Representative western blot (1:1000) with Anti-GSK3α using 20 ng of GST-tagged human recombinant GSK3α and GSK3β.

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