Description: Recombinant human CBP (1319-1710) was expressed in E. coli cells using a N-terminal GST tag.

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Western Blot

Molecular Weight: ~71 kDa

Gene Aliases: CREBBP, RTS, RSTS

Scientific Background: CBP or CREB-binding protein is a nuclear transcriptional coactivator protein that binds specifically to the PKA-phosphorylated form of the CREB protein. Microinjection of an anti-CBP antiserum into fibroblasts leads to inhibition of transcription from a cAMP promoter (1). CBP can also cooperates with upstream activators, such as JUN. When JUN is phosphorylated at the transcriptionally stimulatory sites ser73 and ser63, it binds CBP with comparable affinity to CREB. Insulin signaling may directly regulate many cAMP signaling pathways at the transcriptional level by controlling CBP recruitment (2). Mutant CBP can be aberrantly recruited to CREB protein, resulting in inappropriate activation of gluconeogenesis and glucose intolerance.

References: 
1. Arias, J. et al. : Activation of cAMP and mitogen responsive genes relies on a common nuclear factor. Nature 370: 226-229, 1994.

2. Zhong, H. et al: The phosphorylation status of nuclear NF-kappa-B determines its association with CBP/p300 or HDAC-1. Molec. Cell 9: 625-636, 2002.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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