Description: Recombinant full-length human EEF2K was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Storage, Stability, and Shipping: Store product at -70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Kinase Assay, Western Blot

Molecular Weight: ~125 kDa

Gene Aliases: eEF-2K, HSU93850, MGC45041

Scientific Background: EEF2K is a highly conserved calmodulin-dependent protein kinase that links activation of cell surface receptors to cell division. EEF2K is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2), an abundant cytoplasmic protein that catalyzes the movement of the ribosome along mRNA during translation in eukaryotic cells, and inhibits the EEF2 function (1). EEF2K is highly expressed in heart and skeletal muscle, suggesting that EEF2 phosphorylation may be particularly important in muscle. EEF2K is highly expressed in patients with systemic lupus erythematosus (2) as well as in many cancers.

References: 
1. Ryazanov, A. G. et al; Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase. Proc. Nat. Acad. Sci. 94: 4884-4889, 1997.

2. Arora, S. et al; Detection of anti-elongation factor 2 kinase (calmodulin-dependent protein kinase III) antibodies in patients with systemic lupus erythematosus. Biochem. Biophys. Res. Commun. 293: 1073-1076, 2002.

		Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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