Description: Recombinant full-length human p110 delta and human p85 alpha were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Kinase Assay, Western Blot

Molecular Weight: ~111 kDa (p110δ), ~86 kDa (p85α)

Gene Aliases : p110δ: p110δp85α: PIK3R1, GRB1, p85-ALPHA

Scientific Background: PI3K (p110δ/p85α) is a member of the PI3K family that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring (1). PI3K (p110δ/p85α) plays a role in oncogenic transformation, and is consistently expressed at a high level in blast cells from AML. PI3K (p110δ/p85α) can play a selective role in the amplification of phosphatidylinositol (3,4,5) trisphosphate (PIP3) levels that lead to neutrophil polarization, and regulates directional migration, but not random locomotion (2). PI3K (p110δ/p85α) signaling pathway is involved in immune responses relevant to the pathogenesis of rheumatoid arthritis and other inflammatory diseases.

References: 
1. Vanhaesebroeck, B. et.al: p110-delta, a novel phosphoinositide 3-kinase in leukocytes. Proc. Nat. Acad. Sci. 94: 4330-4335, 1997.

2. Chantry, D. et.al: p110-delta, a novel phosphatidylinositol 3-kinase catalytic subunit that associates with p85 and is expressed predominantly in leukocytes. J. Biol. Chem. 272: 19236-19241,1997

		Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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