Storage, Stability and Shipping
: Store product at –70o
C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
: Kinase Assay, Western Blot
: ~82 kDa
: c-ros-1; MCF3; ROS
: ROS1 is a proto-oncogene and member of the sevenless subfamily of tyrosine kinase insulin receptor genes. ROS1 is highly-expressed in a variety of tumor cell lines and functions as a growth or differentiation factor receptor. The FIG gene can fuse with the ROS1 gene in glioblastoma cell lines (1). The resulting ROS1/FIG fusion protein is a constitutively activated tyrosine kinase. Direct interaction of ROS1 and the phosphatase SHP-1 can lead to efficient downregulation of ROS1-mediated signaling (2). Binding sites in the ROS1 cytoplasmic domain display high affinity binding to the SHP-1 N-terminal SH2 domain.
1. Charest, A. et al: Fusion of FIG to the receptor tyrosine kinase ROS in a glioblastoma with an interstitial del (6) (q21q21). Genes Chromosomes Cancer 37: 58-71, 2003.
2. Biscup, C. et al: Visualization of SHP-1-target interaction. J Cell Sci. 2004 Oct 1; 117(Pt 21):5165-78.
: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
||Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
||Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.
The ROS1, Active product can be utilized in the following research areas, but not limited to:
Cancer, Metabolic Disorder, Receptor Tyrosine Kinases