: Store at 4°C (add 0.1% NaN3
) for several months, and at -20°C for longer periods.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
: Recognizes the PAK1 protein
: Western blot PAK1 from human and mouse cells
PAK1 from other species may also be detectable
: Synthetic peptide corresponding to amino acids 210-214 of human PAK1
: PBS pH 7.4, 0.02% sodium azide, 50% glycerol
: Affinity chromatography
1. Jaffer, Z M. et al: p21-activated kinases: three more join the Pak. Int J Biochem Cell Biol. 2002 Jul;34(7):713-7.
2. Parrini, M C. et al: Spatiotemporal regulation of the Pak1 kinase. Biochem Soc Trans. 2005 Aug;33(Pt 4):646-8.
: PAK1 is a member of the p21-activated kinases (PAKs) which have been implicated in the regulation of cell morphology, motility and transformation. These serine/threonine kinases are activated by and are effectors of small GTPases, CDC42 and RAC. PAK1 belongs to the Group I PAKs which also includes PAK2 and PAK3 (1). PAK1 is a key regulator of the actin cytoskeleton, adhesion and cell motility. Inactive dimeric PAK1 is mainly cytosolic and interaction with the activators Cdc42-GTP and Rac1-GTP stimulates the kinase at the sites of cellular protrusions forming adhesions to the extracellular matrix (2).
||Sample Data: Representative western blot with Anti-PAK1 (1:1000) using 20 ng of human recombinant PAK1, PAK2, PAK3, PAK4, and PAK7.
||Sample Data: Representative western blot with Anti-PAK1 (1:1000) using 3 μg, 10 μg, and 30 μg of Jurkat cel lysate.
The Anti-PAK1 product can be utilized in the following research areas, but not limited to:
Cancer, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases