Description: Rabbit Polyclonal Antibody

References: 
1. Manicassamy, S. and Sun, Z. The critical role of protein kinase C-theta in Fas/Fasligand-mediated apoptosis. J. Immunol. 2007; 178(1):312-9.

2. Kim, J K. et al: PKC-theta knockout mice are protected from fat-induced insulin resistance. J. Clin. Invest. 2004; 114(6):823-7.

Scientific Background: Protein kinase C theta (PKCθ) is important component in the intracellular signaling cascade (1). Recent studies have suggested that local accumulation of fat metabolites inside skeletal muscle may activate a serine kinase cascade involving PKC-theta leading to defects in insulin signaling and glucose transport in skeletal muscle (2). Insulin resistance plays a primary role in the development of type 2 diabetes and may be related to alterations in fat metabolism. PKC-theta is a crucial component mediating fat-induced insulin resistance in skeletal muscle and is a potential therapeutic target for the treatment of type 2 diabetes (2).

Immunogen: Synthetic peptide corresponding to amino acids 674-678 of human PKCθ

Formulation: PBS pH 7.4, 0.02% sodium azide, 50% glycerol

Purification: Affinity chromatography

Stability: Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Specificity: Recognizes the PKCθ protein

Cited Applications: WB

Cross Reactivity: Western blot human PKCθ

PKCδ/θ from other species may also be detectable

Host: Rabbit

		Sample Data:  Representative western blot with Anti- PKCθ (1:1000) using 20ng of GST –tagged human recombinant PKCα, PKCβI, PKCβII, PKCγ, PKCδ, PKCε, PKCη, PKCθ, PKCμ, PKCν, PKCζ, and PKCι.

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