Description: Rabbit Polyclonal Antibody

Specificity: Anti-RSK1 antibody recognizes the RSK1 protein.

Cited Applications: WB

Cross Reactivity: RSK1 from Human, Mouse, Rat

RSK1 from other species may also be detectable.

Host: Rabbit

Immunogen: C-LAQRRVRKLPSTTL peptide KLH conjugates

Formulation: TBS at pH 7.2, 50% glycerol

Purification: Affinity purified by peptide C-LAQRRVRKLPSTTL on agarose from rabbit serum

Stability: Store at 4°C (add 0.1% NaN₃) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background: RSK1 is a member of the RSK (ribosomal S6 kinase) family that are growth factor-regulated serine/threonine kinases. RSK1 contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signaling pathway. RSK1 encodes a predicted 735-amino acid protein containing 2 distinct consensus ATP-binding site sequences. RSK1 transcript is present in lymphocytes, skeletal muscle, liver, and adipose tissue (1). RSKs are implicated in the activation of the mitogen-activated kinase (MAPK) cascade and the stimulation of cell proliferation and differentiation (2).

References: 
1. Moller, D E. et al: Human rsk isoforms: cloning and characterization of tissue-specific expression. Am. J. Physiol. 266: C351-C359, 1994.

2. Gross, S D. et al: Induction of metaphase arrest in cleaving Xenopus embryos by the protein kinase p90(Rsk). Science 286: 1365-1367, 1999.

		Sample Data:  Representative western blot (1:2000) using 5µg, 10µg, and 20µg of from HeLa cell lysate.

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