Description: Full length recombinant human CK1 gamma 3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Kinase Assay, Western Blot

Molecular Weight: ~73 kDa

Gene Aliases : CSNK1G3

Scientific Background: CK1gamma3 is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. CK1gamma3 is a ubiquitously expressed protein kinase found in the nuclei, cytoplasm and membrane fractions of eukaryotic cells (1). CK1gamma3 preferentially phosphorylates acidic substrates using ATP as a phosphate donor. The kinase domain of CKI isoforms have been shown to associate with protein kinase C-potentiated inhibitor protein of 17kDa, called CPI-17 (2). CPI-17 specifically inhibits myosin light chain phosphatase and this effect is potentiated when it is phosphorylated on Thr-38 by protein kinases such as the CK1 isoforms.

References: 
1. Kusuda, J. et al: Cloning and chromosome mapping of the human casein kinase I gamma-3 gene (CSNK1G3). Cytogenet. Cell Genet. 83: 101-103, 1998.

2. Zemlickova, E. et al: Association of CPI-17 with protein kinase C and casein kinase I. Biochem Biophys Res Commun. 2004 Mar 26;316(1):39-47.

		Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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