Storage, Stability and Shipping
: Store product at –70o
C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
: Kinase Assay, Western Blot
: ~71 kDa
: MAPK10; JNK3A, MGC50974, PRKM10, p493F12, p54bSAPK; FLJ12099, FLJ33785
: JNK3 is a member of the c-Jun N-terminal kinases (JNKs) which are part of the mitogen-activated protein (MAP) kinase family, and regulate signal transduction in response to environmental stress. JNK3 phosphorylates various transcription factors such as ATF2, Elk-1 and members of the Jun family (1). Activation and nuclear localization of JNK3, a neuronal-specific isoform of JNK, has been associated with hypoxic and ischemic damage of CA1 neurons in the hippocampus. Knockout mice lacking JNK3 showed reduced apoptosis of hippocampal neurons and reduced seizure induced by kainic acid, a glutamate-receptor agonist (2).
1. Gupta, S. et al: Selective interaction of JNK protein kinase isoforms with transcription factors. EMBO J. 1996 Jun 3; 15(11):2760-70.
2. Yang, D D. et al: Absence of excitotoxicity-induced apoptosis in the hippocampus of mice lacking the Jnk3 gene. Nature. 1997 Oct 23; 389(6653):865-70.
: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
||Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
||Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.
The JNK3, Active product can be utilized in the following research areas, but not limited to:
Apoptosis/Autophagy, Cardiovascular Disease, Cellular Stress, Inflammation, JNK/SAPK Pathway, Neurobiology, NfkB Pathway, Ser/Thr Kinases