PI3K (p110 alpha/p85 alpha) Active Enzymes Active Kinases


Home › Enzymes - Active Kinases › PI3K (p110 alpha/p85 alpha), Active

PI3K (p110 alpha/p85 alpha), Active (P27-18H)
Catalog Number Pack Size Price (USD)  
PI3K (p110 alpha/p85 alpha), Active P27-18H-05 5 ug $145   PI3K (p110 alpha/p85 alpha), Active Add to Cart
PI3K (p110 alpha/p85 alpha), Active P27-18H-10 10 ug $205   PI3K (p110 alpha/p85 alpha), Active Add to Cart
PI3K (p110 alpha/p85 alpha), Active P27-18H-BULK BULK CONTACT US  
* Prices listed in the "shopping cart" are not discount inclusive. Promotional Prices will be applied internally during the processing of orders and net totals adjusted accordingly.

Bulk Pricing for PI3K (p110 alpha/p85 alpha), Active

Product Data Sheet(s)




Description: Recombinant full-length mouse p110alpha and human p85alpha were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Tag: His tags
Expression System: Sf9 insect cells using baculovirus
Species: Mouse and Human
Sequence: Full length
Genbank Number: NM_008839 , NM_181523
   

Storage, Stability, and Shipping: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Kinase Assay, Western Blot

Molecular Weight: ~111 kDa (p110α), ~86 kDa (p85α)

Gene Aliases : p110α: PIK3CA, PI3K, p110-alpha p85α: PIK3R1, GRB1, p85-ALPHA

Scientific Background: The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110α catalytic subunit (PIK3CA) is frequently mutated or amplified in a variety of cancers including ovarian and colon (1). PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of p110α is essential for vascular development and inactivation of p110α leads to severe defects in angiogenic sprouting and vascular remodeling (2).

References: 
1. Samuels, Y. et al: High frequency of mutations of the PIK3CA gene in human cancers. Science 304: 554 only, 2004.

2. Graupera, M.et al: Angiogenesis selectively requires the p110-alpha isoform of PI3K to control endothelial cell migration. Nature 453: 662-666, 2008.



  Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.


  Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.




PI3K (p110 alpha/p85 alpha), Active Research Areas

The PI3K (p110 alpha/p85 alpha), Active product can be utilized in the following research areas, but not limited to:

AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Lipid Kinases, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling



PI3K (p110 alpha/p85 alpha), Active Related Products Found: Total 36 Related Products
Product Type Product Name Catalog Number Application Pack Sizes
Enzymes PI3K (p110 beta/p85 alpha), Active P28-10H Kinase Assay, Western Blot View PI3K (p110 beta/p85 alpha), Active
Enzymes PDK1, Active P14-10H Kinase Assay, Western Blot View PDK1, Active
Enzymes AKT1, Active A16-10G Kinase Assay, Western Blot View AKT1, Active
Signaling Proteins AKT1, Unactive A16-14G Kinase Assay, Western Blot View AKT1, Unactive
Enzymes AKT2, Active A17-10G Kinase Assay, Western Blot View AKT2, Active
Enzymes AKT2, Active A17-10H Kinase Assay, Western Blot View AKT2, Active
Enzymes AKT3, Active A18-10G Kinase Assay, Western Blot View AKT3, Active
Signaling Proteins AKT3, Unactive A18-14G Kinase Assay, Western Blot View AKT3, Unactive
Enzymes PDGFR alpha, Active P12-11G Kinase Assay, Western Blot View PDGFR alpha, Active
Enzymes PDGFR beta, Active P13-11G Kinase Assay, Western Blot View PDGFR beta, Active
Enzymes InsR, Active I08-11G Kinase Assay, Western Blot View InsR, Active
Enzymes IGF1R, Active I02-11H Kinase Assay, Western Blot View IGF1R, Active
Enzymes FLT1, Active F11-11G Kinase Assay, Western Blot View FLT1, Active
Enzymes FLT3, Active F12-11G Kinase Assay, Western Blot View FLT3, Active
Enzymes FGR, Active F10-10G Kinase Assay, Western Blot View FGR, Active
Enzymes HER2, Active E27-11G Kinase Assay, Western Blot View HER2, Active
Signaling Reagents Kinase Assay Buffer I K01-09 Kinase Assay View Kinase Assay Buffer I
Signaling Reagents Kinase Dilution Buffer III K23-09 Kinase Assay View Kinase Dilution Buffer III
Signaling Proteins PTEN Protein P23-34G Western Blot View PTEN Protein
Signaling Proteins PI3K (p85 alpha) Protein P31-30H Kinase Assay, Western Blot View PI3K (p85 alpha) Protein
Enzymes PI3K (p110 alpha(E545K)/p85 alpha), Active P27-15H Kinase Assay, Western Blot View PI3K (p110 alpha(E545K)/p85 alpha), Active
Enzymes PI3K (p110 delta/p85 alpha), Active P30-10H Kinase Assay, Western Blot View PI3K (p110 delta/p85 alpha), Active
Enzymes PI3K (p120 gamma), Active P29-10H Kinase Assay, Western Blot View PI3K (p120 gamma), Active
Enzymes PI3K (p110 alpha/p85 alpha), Active P27-10H Kinase Assay, Western Blot View PI3K (p110 alpha/p85 alpha), Active
Enzymes PI4K2A, Active P21-10G Kinase Assay, Western Blot View PI4K2A, Active
Enzymes PI4K2B, Active P22-10G Kinase Assay, Western Blot View PI4K2B, Active
Enzymes PI4KB, Active P32-10G Kinase Assay, Western Blot View PI4KB, Active
Enzymes PIP5K1A, Active P16-10AG Kinase Assay, Western Blot View PIP5K1A, Active
Enzymes PIP5K1C, Active P16-10CG Kinase Assay, Western Blot View PIP5K1C, Active
Enzymes PI3K (p110 beta/p85 beta), Active P28-10BH Kinase Assay, Western Blot View PI3K (p110 beta/p85 beta), Active
Signaling Proteins PI3K (p85 beta) Protein P31-30BH Kinase Assay, Western Blot View PI3K (p85 beta) Protein
Enzymes PIK3C3 (Vps34), Active P26-10G Kinase Assay, Western Blot View PIK3C3 (Vps34), Active
Enzymes PIK3C2G, Active P25-11G Kinase Assay, Western Blot View PIK3C2G, Active
Signaling Proteins PI3K (p55 gamma) Protein P31-30CH Western Blot View PI3K (p55 gamma) Protein
Signaling Proteins PI3K (p65 alpha) Protein P31-31H Western Blot View PI3K (p65 alpha) Protein
Enzymes PI3K (p110 alpha/p55 gamma), Active P27-10CH Kinase Assay, Western Blot View PI3K (p110 alpha/p55 gamma), Active


Items in Your Cart (0)
Product Types
Enzymes
Enzymes Active Acetyltransferases Active Acetyltransferases
Enzymes Active Arginine Deiminases Active Arginine Deiminases
Enzymes Active Histone Deacetylases Active Histone Deacetylases
Enzymes Active Kinases Active Kinases
Enzymes Active Methyltransferase Active Methyltransferase
Enzymes Active Phosphatases Active Phosphatases
Enzymes Active Phosphodiesterases Active Phosphodiesterases
Extracellular Ligands
Extracellular Ligands Chemokines Chemokines
Extracellular Ligands Cytokines Cytokines
Extracellular Ligands Growth Factors Growth Factors
Signaling Proteins
Signaling Proteins Acetyl/Methyltransferase Proteins Acetyl/Methyltransferase Proteins
Signaling Proteins Adaptor Proteins Adaptor Proteins
Signaling Proteins Apoptosis Proteins Apoptosis Proteins
Signaling Proteins Arginine Deiminases Arginine Deiminases
Signaling Proteins Cell Cycle Proteins Cell Cycle Proteins
Signaling Proteins Cell Stress & Chaperone Proteins Cell Stress & Chaperone Proteins
Signaling Proteins Cellular Proteins Cellular Proteins
Signaling Proteins Deacetylase/Demethylase Proteins Deacetylase/Demethylase Proteins
Signaling Proteins Fructose Kinase Proteins Fructose Kinase Proteins
Signaling Proteins G-Proteins G-Proteins
Signaling Proteins Lysyl Oxidase Proteins Lysyl Oxidase Proteins
Signaling Proteins Methylcytosine Dioxygenase Proteins Methylcytosine Dioxygenase Proteins
Signaling Proteins Microtubule/Actin Associated Proteins Microtubule/Actin Associated Proteins
Signaling Proteins Tau Proteins Tau Proteins
Signaling Proteins Transcription Proteins Transcription Proteins
Signaling Proteins Ubiquitin Proteins Ubiquitin Proteins
Signaling Proteins Unactive Kinases Unactive Kinases
Signaling Reagents
Signaling Reagents Assay Reagents Assay Reagents
Signaling Reagents Oligo Substrates Oligo Substrates
Signaling Reagents Peptide Substrates Peptide Substrates
Signaling Reagents Protein Substrates Protein Substrates
Antibodies
Antibodies Isoform Specific Antibodies Isoform Specific Antibodies
Antibodies Modification Antibodies Modification Antibodies
Antibodies Phospho Specific Antibodies Phospho Specific Antibodies
Antibodies Primary Antibodies Primary Antibodies
Antibodies Secondary Antibodies Secondary Antibodies
Antibodies Tag Antibodies Tag Antibodies
Discovery Services
Compound Selective Profiling Compound Selective Profiling
Custom Protein Development Custom Protein Development

Research Categories
AKT/PKB Pathway AKT/PKB Pathway
Angiogenesis Angiogenesis
Apoptosis/Autophagy Apoptosis/Autophagy
Cancer Cancer
Cardiovascular Disease Cardiovascular Disease
Cell Cycle Cell Cycle
Cellular Stress Cellular Stress
Cytoplasmic Tyrosine Kinases Cytoplasmic Tyrosine Kinases
ERK/MAPK Pathway ERK/MAPK Pathway
Inflammation Inflammation
Invasion/Metastasis Invasion/Metastasis
JAK/STAT Pathway JAK/STAT Pathway
JNK/SAPK Pathway JNK/SAPK Pathway
Lipid Kinases Lipid Kinases
Metabolic Disorder Metabolic Disorder
Neurobiology Neurobiology
NfkB Pathway NfkB Pathway
p38 Pathway p38 Pathway
Phosphatases Phosphatases
Phosphodiesterases Phosphodiesterases
PKA/PKC Pathway PKA/PKC Pathway
Receptor Tyrosine Kinases Receptor Tyrosine Kinases
Ser/Thr Kinases Ser/Thr Kinases
WNT Signaling WNT Signaling




Keep in touch, join us on:
PI3K (p110 alpha/p85 alpha), Active Enzymes Active Kinases    PI3K (p110 alpha/p85 alpha), Active Enzymes Active Kinases    PI3K (p110 alpha/p85 alpha), Active Enzymes Active Kinases