Description: Recombinant human ATF2 (1-254) was expressed in E.coli cells using a N-terminal GST tag.

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Kinase Assay, Western Blot

Molecular Weight: ~55 kDa

Gene Aliases : HB16; CREB2; TREB7; CRE-BP1; MGC111558

Scientific Background: Activating transcription factor (ATF)-2, also designated CRE-BP1, is a member of the ATF/cyclic AMP-responsive element binding protein family of transcription factors. ATF2 has two unique characteristics: it mediates the adenovirus E1A-induced trans-activation and forms a heterodimer with c-Jun (1). ATF2 is a target of the JNK signal transduction pathway (2). ATF2 is phosphorylated by JNK on two closely spaced threonine residues within the NH2-terminal activation domain. The replacement of these phosphorylation sites with alanine inhibited the transcriptional activity of ATF2. Furthermore, p38 MAPK can also phosphorylate ATF2.

References: 
1. Nomura, N. et al: Isolation and characterization of a novel member of the gene family encoding the cAMP response element-binding protein CRE-BP1.J Biol Chem. 1993 Feb 25;268(6):4259-66.

2. Gupta, S. et al: Transcription factor ATF2 regulation by the JNK signal transduction pathway. Science. 1995 Jan 20;267(5196):389-93.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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