Description: Recombinant full-length human CAMKK1 was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Kinase Assay, Western Blot

Molecular Weight: ~94 kDa

Gene Aliases : CAMKKA, MGC34095, DKFZp761M0423

Scientific Background: CAMKK1 or CAMKKα is a Ca(2+)/calmodulin-dependent protein kinase that activates CaM-kinases I and IV via phosphorylation of their Thr(177) and Thr(196) residues, respectively. Recent studies have shown that the activity of CAMKK1 is decreased upon phosphorylation by cAMP-dependent protein kinase (PKA) (1) The CAMKKalpha has been identified in intact cells as AMPKKs, predicting a significant role for this kinase in regulating AMPK activity in vivo. It has been shown that 2-deoxyglucose- and ionomycin-stimulated AMPK activity is substantially reduced in HeLa cells transfected with small interfering RNAs specific for CAMKKα (2).

References: 
1. Okuno, S. et al: Regulation of Ca(2+)/calmodulin-dependent protein kinase kinase alpha by cAMP-dependent protein kinase: I. Biochemical analysis. J Biochem (Tokyo). 2001 Oct;130(4):503-13.

2. Hurley, R L. et al: The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases. J Biol Chem. 2005 Aug 12;280(32):29060-6.

		Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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