Description: Recombinant human full length PDGF was expressed in E. coli cells and purified by proprietary chromatographic techniques.

Formulation: Recombinant protein stored in 1 X PBS, 10% glycerol.

Storage, Stability, and Shipping: Stored at desiccated below -20^oC. It is stable at 4^oC between 2-7 days. For long term storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Cell Assay

Endotoxin Level: Less than 0.1 ng/µg (IEU/µg) of PDGF.

Molecular Weight: ~17 kDa

Gene Aliases: PDGF1; PDGFA; PDGF-A

Scientific Background: PDGF is a member of the platelet-derived growth factor family which is mitogenic factors for cells of mesenchymal origin and is characterized by a motif of eight cysteines. PDGF is a cationic glycoprotein consisting of homo- or heterodimers of polypeptide chains A and/or B and a potent mitogen for connective tissue cells and exerts its function by interacting with 2 related receptor tyrosine kinases, PDGFRA and PDGFRB (1). PDGF A and B chain mRNA in mesenchymal-appearing intimal cells and endothelial cells, respectively, of atherosclerotic plaques. Moreover PDGF receptor mRNA was localized predominantly in the plaque intima (2).

References: 
1. Bonthron, D. et.al: Platelet-derived growth factors A chain: confirmation of localization of PDGFA to chromosome 7p22 and description of an unusual minisatellite. Genomics 13: 257-263, 1992.

2. Wilcox, J. N.et.al: Platelet-derived growth factor mRNA detection in human atherosclerotic plaques by in situ hybridization. J. Clin. Invest. 82: 1134-1143, 1988.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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