Description: Recombinant human MEKK6 (620-950) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Kinase Assay, Western Blot

Molecular Weight: ~67 kDa

Gene Aliases: MAP3K6, MAPKKK6, ASK2

Scientific Background: MEKK6 or MAP3K6 (mitogen-activated protein kinase kinase kinase 6) is a member of the serine/threonine protein kinase family. MEKK6 was identified by its interaction with MAP3K5/ASK, a protein kinase and an activator of c-Jun kinase (MAPK7/JNK) and MAPK14/p38 kinase (1). MEKK6 cascades relay signals from the cell surface to the nucleus which result in gene transcription (2). MEKK6 activates the JNK and p38 pathway and induces apoptosis when overexpressed in stably transfected cells. MEKK6 transcript is expressed in several tissues, with the highest expression in heart and skeletal muscle.

References: 
1. Wang, X. S. et al: MAPKKK6, a novel mitogen-activated protein kinase kinase kinase, that associates with MAPKKK5. Biochem. Biophys. Res. Commun. 253: 33-37, 1998.

2. Takeda, K. et al: Apoptosis signal-regulating kinase (ASK) 2 functions as a mitogen-activated protein kinase kinase kinase in a heteromeric complex with ASK1. J Biol Chem. 2007 Mar 9;282(10):7522-31.

		Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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