Description: Recombinant full-length human SLK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Kinase Assay, Western Blot

Molecular Weight: ~180 kDa

Gene Aliases: LOSK, STK2, se20-9, KIAA0204, MGC133067, bA16H23.1

Scientific Background: SLK or LOSK is a Ste20-like protein kinase that associates with microtubule and centrosome. Inhibition of SLK activity by dominant-negative mutant or RNAi leads to unfocused microtubule arrangement indicating that SLK is needed for microtubule organization and for the proper localization of Golgi complex (1). Expression of v-Src can down-regulate SLK activity through the involvement of CK2 which can directly phosphorylate and inhibit SLK activity whereas inhibition of CK2 in v-Src-transformed cells results in normal SLK activity (2). CK2 and SLK can be co-localized in fibroblasts spreading on fibronectin-coated substrates.

References: 
1. Burakov A V, et al: LOSK (SLK) protein kinase activity is necessary for microtubule organization in the interphase cell centrosome. Dokl Biol Sci. 2005;403:317-9

2. Chaar Z, et al: v-Src-dependent down-regulation of the Ste20-like kinase SLK by casein kinase II. J Biol Chem. 2006 Sep 22;281(38):28193-9.

		Specific Activity:  Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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