Description: Recombinant full length human RhoD was expressed in E. coli cells using an N-terminal His tag.

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Kinase Assay, Western Blot

Molecular Weight: ~22 kDa

Gene Aliases : Rho; RHOHP1; RHOM

Scientific Background: RhoD is a member of the Rho gene family that interacts with protein kinases and serves as a target of activated GTPase. Rho binds GTP and plays a critical role in muscle differentiation by regulating the expression of myogenin and MEF2 genes. Furthermore, Rho is involved in endosome dynamics and reorganization of the actin cytoskeleton and plays a role in coordinating membrane transport (1). mDia and ROCK are downstream effectors of Rho mediating Rho action on the actin cytoskeleton; mDia produces actin filaments by nucleation and polymerization and ROCK activate myosin to cross-link them for induction of actomyosin bundles and contractility (2).

References: 
1. Gasman, S. et al: RhoD regulates endosome dynamics through Diaphanous-related Formin and Src tyrosine kinase. Nature Cell Biol. 5: 195-204, 2003.

2. Narumiya, S. et al: Rho signaling, ROCK and mDia1, in transformation, metastasis and invasion. Cancer Metastasis Rev. 2009 Jun;28(1-2):65-76.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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