Description: Recombinant full-length human CDK4 was expressed in E. coli cells using a N-terminal GST tag.

Storage, Stability, and Shipping: Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications: Kinase Assay, Western Blot

Molecular Weight: ~57 kDa

Gene Aliases : CMM3, MGC14458, PSK-J3

Scientific Background: CDK4 is a member of the cyclin-dependent protein kinases and is involved in the control of cell proliferation during the G1 phase of the cell cycle. CDK4 forms a complex with the D-type cyclins which regulate its activity. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 can mediate phosphorylation of the C-terminal region of RB protein leading to an active transcriptional repression of E2F complex (1). CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. Pharmacologic inactivation of CDC37/HSP90 function leads to reduced stability of CDK4. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2 (2).

References: 
1. Harbour, J W. et al: Cdk phosphorylation triggers sequential intramolecular interactions that progressively block Rb functions as cells move through G1. Cell 98: 859-869, 1999.

2. Matsuura, I. et al: Cyclin-dependent kinases regulate the antiproliferative function of Smads. Nature 430: 226-231, 2004.

		Purity:  Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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