Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies
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Frequently Asked Questions

 
   
 
Technical Questions
 
For all of our products we run SDS-PAGE gel with a titration of the enzyme, followed by the densitometry measurement to determine the purity.
To request the sequence information for the protein of interest please contact us.
An enzyme titration curve and calculated specific activity are included in the data sheet of each active enzyme. It is recommend that the researchers perform a serial dilution of an enzyme product to determine the optimal amount to use in their own assay systems.
The concentration of each of our products is calculated from a titration on SDS-PAGE gel with a standard curve of the Bovine serum albumin (BSA) or the Bovine gamma globulin (BGG).
Yes, we may provide enzymes at a higher concentration. Please contact us to obtain more detailed information.
Yes, we can supply our protein in a customized buffer formulation. Please contact us to submit your request.
SignalChem’s active enzymes are produced using specific treatment or conditions to achieve optimal activity in vitro, while unactive proteins are generally produced without any stimulation or from prokaryotic systems so the enzymes only show minimal basal activity. Active enzymes can be directly used in biochemical assays for compound screening or enzymatic studies. Unactive proteins can be used as substrates, negative controls, or to study activation of the particular enzymes in vitro.
SignalChem’s active enzymes are activated using specific protocols during the process of expression and production to achieve optimal post-translation modification and in vitro activity. The treatment methods may vary for different enzymes depending on their functional roles.
Yes, we do customize the proteins with other tags (e.g. FLAG, Fc). Please contact us for the detailed information.
Yes, we can customize the protein as per your requirements. Please contact us to submit your query.
Please contact us to obtain the details for tag cleavage site.
Expression system is chosen based on the function and application of each product.
SignalChem’s active enzymes are validated for in vitro activity assays. We offer a wide range of custom services including additional purification steps, buffer exchange, and tag modification to fulfill the requirements for other applications such as protein crystallography. Please contact us find more information about this service.
SignalChem’s active enzymes and recombinant proteins both may work in binding assays. We recommend clients to test the compatibility of a product in their assay system. Contact us to request a free sample.
SignalChem offers Profiling Services for enzymatic studies including Km determination. Please click here to find more information about this service.
Generally active kinases are phosphorylated on certain sites in their catalytic domain. However, the number of total phosphorylation sites from each active kinase may vary. We do not perform any test to probe for specific phosphorylation in our kinases.
Phosphorylation of a certain residue can be determined by Western Blot or ELISA using phospho-specific antibodies.
 
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Still have questions?


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