Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Overview:

The PAD4 Inhibitor Screening Kit is a direct Enzyme-Linked ImmunoSorbent Assay (ELISA) kit for use in PAD4-targeted inhibitor profiling assays. The kit includes recombinant full-length human PAD4 expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag (accession number BC025718).

Components:

	Part Number	Part	Size	Storage
Kit Box	CP01-506	NeutrAvidin Coated Plate	1 plate/1	Store kit box and contents at 4^oC.
	AB99-61DM	Detection Antibody, 500X	25 ul/1
	CS01-506	TMB Substrate	6 ml/1
	P312-09	PAD Buffer	8 ml/1
	SS01-09	Stop Solution	3 ml/1
	T01-09	Trypsin Digestion Buffer	15 ml/1
	WB20-09	Wash Buffer, 20X	15 ml/2
Ice Pack	D86-09	DTT Solution	10 ul/1	Store product at –20^oC or –70^oC as specified on individual component labels. For PAD enzymes, avoid repeated handling and multiple freeze/thaw cycles.
	T575-31N	Trypsin, Active	200 ug/1
	P312-58	PAD Substrate	500 ug/1
	P312-37C	PAD Cocktail, Active	5 ug/1
	P312-310DG	PAD4, Active	50 ug/1

References:

1. Rothnagel, J.A., & Rogers, G.E. (1984). Citrulline in proteins from the enzymatic deimination of arginine residues. Methods Enzymol. 107, 624-31.

2. Raijmakers, R. et al. (2007). Methylation of Arginine Residues Interferes with Citrullination by Peptidylarginine Deiminases in vitro. J. Mol. Biol. 367, 1118–29.

3. Tilvawala, R., & Thompson, P.R. (2019). Peptidyl arginine deiminases: detection and functional analysis of protein citrullination. Curr. Opin. Struct. Biol. 20, 1-11.

4. Wildeman E., & Pires M.M. (2013). Facile fluorescence-based detection of PAD4-mediated citrullination. Chembiochem. 14, 963-7.

5. Clancy K.W. et al. (2016). Detection and identification of protein citrullination in complex biological systems. Curr. Opin. Chem. Biol. 30, 1-6.

Representative Data:

Representative Data Demonstration of high sensitivity and specificity of the assay achieved by using human recombinant PAD enzymes with PAD Activity Kit.

Related Products:

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
PAD Detection Kit	P312-863
PAD Cocktail Inhibitor Screening Kit	P37C-863
PAD2 (human) Inhibitor Screening Kit	P318BG-863
PAD1 Inhibitor Screening Kit	P310G-863
PAD1, Active	P312-310G	Human	GST	Sf9 insect cells using baculovirus	Full length	NM_013358
PAD2, Active	P312-310BG	Mouse	GST	Sf9 insect cells using baculovirus	Full length	BC049947
PAD2, Active	P312-318BG	Human	GST	Sf9 insect cells using baculovirus	Full length	NM_007365
PAD3, Active	P312-310CG	Human	GST	Sf9 insect cells using baculovirus	Full length	NM_016233
PAD4, Active	P312-310DG	Human	GST	Sf9 insect cells using baculovirus	Full length	BC025718
PAD2 (mouse) Inhibitor Screening Kit	P310BG-863
PAD3 Inhibitor Screening Kit	P310CG-863
PAD4 Inhibitor Screening Kit	P310DG-863

Assay Workflow:

Technical Manual:

CoA Sheet:

SDS:

There are no related publications available for this product.

PAD4 Inhibitor Screening Kit

Shop Online

Subscribe to our mailing list!