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2019-nCoV N-S1 Human IgG-IgA-IgM ELISA Kit

Catalog No. C19NS1-P877


$620.00
96 Reactions Per Kit

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Overview:

The 2019-nCoV N-S1 Human IgG-IgA-IgM ELISA Kit is an enzyme-linked immunosorbent assay for the pan-specific detection of Human anti-2019-nCoV N Protein & Spike Protein S1 IgG-IgA-IgM in serum or other biological samples. Based on indirect ELISA principles, the 2019-nCoV N-S1 Human IgG-IgA-IgM ELISA employs recombinant 2019-nCoV N Protein - Spike Protein S1 RBD Chimera coated on a 96- well plate. Controls and samples (with necessary dilution) are pipetted into the wells; the target IgG-IgA-IgM in the controls and samples binds to the immobilized protein chimera. The intensity of color development in the wells is proportional to the amount of target IgG-IgA-IgM bound.


Components:

SignalChem Cat# Component Name Size Storage
WB20-09Wash Buffer, 20X15 ml x 2 vialsStore kit and contents at 4oC for up to 6 months. Store unused multi well strips in the provided resealable bag and use within one month after opening.
C19NS1P-POSnCoV N-S1 IgG-IgA-IgM POS Control1 ml x 1 vial
C19NS1P-NEGnCoV N-S1 IgG-IgA-IgM NEG Control1 ml x 1 vial
SS01-09Stop Solution6 ml x 1 vials
E02-09nCoV Ig Sample Dilution Buffer10 ml x 2 vials
CS02-506One-Step TMB Substrate10 ml x 1 vials
CP04-C19NSD2019-nCoV RBD-N Protein Chimera Coated Plate1 plate
H38P-63DGAnti-Human IgG-IgA-IgM Fc Detection Antibody, 1X10 ml x 1 vials

References:


1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.

2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.

3. Long Q, et al: Antibody responses to SARS-CoV-2 in patients with COVID-19 Quan-Xin Long. Nature Medicine. 2020, 29 April.

4. Ma H, et al: Serum IgA, IgM, and IgG responses in COVID-19. Cell Mol Immunol. 2020, 17: 773-775.



Representative Data:

Calculation and interpretation of assay results


Related Products:

Name Catalog Number Species Tag Expression System Sequence Genbank Number
2019-nCoV N-S1 Human IgA ELISA KitC19NS1-A877
2019-nCoV N-S1 Human IgG ELISA KitC19NS1-877
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2019-nCoV Spike protein S1, HIS TagC19S1-G241HSARS-CoV-2HISCHO cells16-685MN908947
2019-nCoV Spike protein S1, Fc TagC19S1-G241FSARS-CoV-2FcCHO cells16-685MN908947
2019-nCoV Spike protein S2, Fc TagC19S2-G241FSARS-CoV-2FcCHO cells686-1212MN908947
Anti 2019-nCoV Spike Protein hIgG Antibody, HRP conjugated C19S1-60DHHuman, IgG1CHO cells
Anti 2019-nCoV Spike Protein mIgG AntibodyC19S1-61MHHuman Fab – Mouse IgG FcCHO cells
Anti 2019-nCoV Spike Protein hIgA AntibodyC19S1-A60HHuman, IgACHO cells
Anti 2019-nCoV N-Protein hIgG Antibody, HRP conjugatedC19NP-60DHHuman, IgG1CHO cells
2019-nCoV S1 Protein ELISA KitC19SD-876
2019-nCoV S1 Human IgG ELISA KitC19S1-877
2019-nCoV S1 Human IgG-IgA-IgM ELISA KitC19S1-P877
2019-nCoV N-S1 Human IgM ELISA KitC19NS1-M877
2019-nCoV High Sensitivity S1 Protein ELISA KitC19S1-876


Assay Workflow:


Technical Manual:


CoA Sheet:


SDS:



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