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2019-nCoV N-S1 Human IgA ELISA Kit

Catalog No. C19NS1-A877


$590.00
96 Reactions Per Kit

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Overview:

The 2019-nCoV N-S1 Human IgA ELISA Kit is an enzyme-linked immunosorbent assay for the panspecific detection of Human anti-2019-nCoV N Protein & Spike Protein S1 IgA in serum or other biological samples. Based on indirect ELISA principles, the 2019-nCoV N-S1 Human IgA ELISA employs recombinant 2019-nCoV N Protein - Spike Protein S1 RBD Chimera coated on a 96- well plate. Controls and samples (with necessary dilution) are pipetted into the wells; the target IgA in the controls and samples binds to the immobilized protein chimera. The intensity of color development in the wells is proportional to the amount of target IgA bound.


Components:

SignalChem Cat# Component Name Size Storage
CP04-C19NSD2019-nCoV RBD-N Protein Chimera Coated Plate1 plateStore kit and contents at 4oC for up to 6 months. Store unused multi well strips in the provided resealable bag and use within one month after opening.
WB20-09Wash Buffer, 20X15 ml x 2 vials
C19NS1A-POS-nCoV N-S1 IgA POS Control1 ml x 1 vial
C19NS1A-NEGnCoV N-S1 IgA NEG Control1 ml x 1 vial
SS01-09Stop Solution6 ml x 1 vials
E02-09nCoV Ig Sample Dilution Buffer10 ml x 2 vials
CS02-506One-Step TMB Substrate10 ml x 1 vials
H38A-63DGAnti-Human IgA Fc Detection Antibody, 1X10 ml x 1 vials

References:


1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.

2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.

3. Long Q, et al: Antibody responses to SARS-CoV-2 in patients with COVID-19 Quan-Xin Long. Nature Medicine. 2020, 29 April.

4. Ma H, et al: Serum IgA, IgM, and IgG responses in COVID-19. Cell Mol Immunol. 2020, 17: 773-775.



Representative Data:

Calculation and interpretation of assay results


Related Products:

Name Catalog Number Species Tag Expression System Sequence Genbank Number
2019-nCoV Spike protein RBD, HIS TagC19SD-G241HSARS-CoV-2HISCHO cells319-541MN908947
2019-nCoV Spike protein RBD, Fc TagC19SD-G241FSARS-CoV-2FcCHO cells319-541MN908947
2019-nCoV Spike protein S2, Fc TagC19S2-G241FSARS-CoV-2FcCHO cells686-1212MN908947
Anti 2019-nCoV Spike Protein hIgG Antibody, HRP conjugated C19S1-60DHHuman, IgG1CHO cells
Anti 2019-nCoV Spike Protein mIgG AntibodyC19S1-61MHHuman Fab – Mouse IgG FcCHO cells
Anti 2019-nCoV Spike Protein hIgA AntibodyC19S1-A60HHuman, IgACHO cells
Anti 2019-nCoV N-Protein hIgG Antibody, HRP conjugatedC19NP-60DHHuman, IgG1CHO cells
2019-nCoV S1 Protein ELISA KitC19SD-876
2019-nCoV S1 Human IgG ELISA KitC19S1-877
2019-nCoV S1 Human IgA ELISA KitC19S1-A877
2019-nCoV S1 Human IgG-IgA-IgM ELISA KitC19S1-P877
2019-nCoV N-S1 Human IgG-IgA-IgM ELISA KitC19NS1-P877


Assay Workflow:


Technical Manual:


CoA Sheet:


SDS:



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RELATED PRODUCTS


  2019-nCoV Spike protein RBD, Fc Tag, C19SD-G241F

  2019-nCoV S1 Protein ELISA Kit, C19SD-876

  2019-nCoV S1 Human IgA ELISA Kit, C19S1-A877

  2019-nCoV S1 Human IgG-IgA-IgM ELISA Kit, C19S1-P877


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