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2019-nCoV High Sensitivity S1 Protein ELISA Kit

Catalog No. C19S1-876

96 Reactions Per Kit

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The 2019-nCoV High Sensitivity S1 Protein ELISA Kit v2.0 is a sandwich enzyme-linked immunosorbent assay for the quantitative measurement of 2019-nCoV Coronavirus Spike protein. The S1 Protein ELISA employs a capture anti-S1 protein antibody coated on a 96-well plate. Calibrators and samples are pipetted into the wells; the target protein in the calibrators and samples binds to the immobilized capture antibody. The wells are washed and the HRP-labeled Detection antibody is then added. After washing away the unbound HRP-antibodies, a colorimetric substrate solution (TMB) is pipetted to the wells. The absorbance of the color at 450 nm is measured. The intensity of color development in the wells is proportional to the amount of target protein bound.


SignalChem Cat# Component Name Size Storage
C19S1-HS61DHAnti-nCoV S1 Detection Antibody, HS-1X10 ml x 1 vialStore kit and contents at 4oC for up to 6 months. Store unused multi well strips in the provided resealable bag and use within one month after opening.
CP06-642HLAnti 2019-nCoV Spike Protein Coated Plate1 plate
CS02-506One-Step TMB Substrate10 ml x 1 vial
E01-09nCoV ELISA Sample Dilution Buffer10 ml x 2 vials
SS01-09Stop Solution6 ml x 1 vial
C19SD-CAL50Calibrator 501 ml x 1 vial
WB20-09Wash Buffer, 20X15 ml x 2 vials


1.Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.

2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.

3. Lan J, et al: Crystal structure of the 2019-nCoV Spike receptor-binding domain bound with the ACE2 receptor. bioRxiv. doi: https://doi.org/10.1101/2020.02.19.956235.

Representative Data:

The 2019-nCoV Spike protein concentration of a biological sample may be calculated using the Calibrator by generating a standard curve that correlates the measured O.D. values.

Related Products:

Name Catalog Number Species Tag Expression System Sequence Genbank Number
2019-nCoV Spike protein RBD, Fc TagC19SD-G241FSARS-CoV-2FcCHO cells319-541MN908947
2019-nCoV Spike protein RBD, HIS TagC19SD-G241HSARS-CoV-2HISCHO cells319-541MN908947
2019-nCoV Spike protein S2, Fc TagC19S2-G241FSARS-CoV-2FcCHO cells686-1212MN908947
Anti 2019-nCoV Spike Protein hIgG Antibody, HRP conjugated C19S1-60DHHuman, IgG1CHO cells
2019-nCoV S1 Human IgG-IgA-IgM ELISA KitC19S1-P877
2019-nCoV N-S1 Human IgG-IgA-IgM ELISA KitC19NS1-P877
2019-nCoV Neutralization ELISA KitC19SD-N887

Assay Workflow:

Technical Manual:

CoA Sheet:


There are no related publications available for this product.


  2019-nCoV Spike protein RBD, HIS Tag, C19SD-G241H

  Anti 2019-nCoV Spike Protein hIgG Antibody, HRP conjugated , C19S1-60DH

  2019-nCoV N-S1 Human IgG-IgA-IgM ELISA Kit, C19NS1-P877

  2019-nCoV Neutralization ELISA Kit, C19SD-N887



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