Home   >   Assay Kits    >    PAD Kit    >    PAD2 (mouse) Inhibitor Screening Kit

PAD2 (mouse) Inhibitor Screening Kit

Catalog No. P310BG-863


$750.00
96 Reactions Per Kit

Shop Online





Overview:

The PAD2 Inhibitor Screening Kit is a direct Enzyme-Linked ImmunoSorbent Assay (ELISA) kit for use in PAD2-targeted inhibitor profiling assays. The kit includes recombinant full-length mouse PAD2 expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag (accession number BC049947).


Components:

Part Number Part Size Storage
Kit BoxCP01-506NeutrAvidin Coated Plate1 plate/1Store product at –20oC or –70oC as specified on individual component labels. For PAD enzymes, avoid repeated handling and multiple freeze/thaw cycles.
AB99-61DMDetection Antibody, 500X25 ul/1
P312-09PAD Buffer8 ml/1
CS01-506TMB Substrate6 ml/1
SS01-09Stop Solution3 ml/1
T01-09Trypsin Digestion Buffer15 ml/1
WB20-09Wash Buffer, 20X15 ml/2
Ice PackP312-37CPAD Cocktail, Active5 ug/1
D86-09DTT Solution10 ul/1
T575-31NTrypsin, Active200 ug/1
P312-58PAD Substrate.5 mg/1
P312-310BGPAD2 (mouse), Active100 ug/1

References:


1. Rothnagel, J.A., & Rogers, G.E. (1984). Citrulline in proteins from the enzymatic deimination of arginine residues. Methods Enzymol. 107, 624-31.

2. Raijmakers, R. et al. (2007). Methylation of Arginine Residues Interferes with Citrullination by Peptidylarginine Deiminases in vitro. J. Mol. Biol. 367, 1118–29.

3. Tilvawala, R., & Thompson, P.R. (2019). Peptidyl arginine deiminases: detection and functional analysis of protein citrullination. Curr. Opin. Struct. Biol. 20, 1-11.

4. Wildeman E., & Pires M.M. (2013). Facile fluorescence-based detection of PAD4-mediated citrullination. Chembiochem. 14, 963-7.

5. Clancy K.W. et al. (2016). Detection and identification of protein citrullination in complex biological systems. Curr. Opin. Chem. Biol. 30, 1-6.



Representative Data:

Demonstration of high sensitivity and specificity of the assay achieved by using human recombinant PAD enzymes with PAD Activity Kit.


Related Products:

Name Catalog Number Species Tag Expression System Sequence Genbank Number
PAD Detection KitP312-863
PAD Cocktail Inhibitor Screening KitP37C-863
PAD2 (human) Inhibitor Screening KitP318BG-863
PAD1 Inhibitor Screening KitP310G-863
PAD4 Inhibitor Screening KitP310DG-863
PAD3 Inhibitor Screening KitP310CG-863
PAD1, ActiveP312-310GHumanGSTSf9 insect cells using baculovirusFull lengthNM_013358
PAD2, ActiveP312-310BGMouseGSTSf9 insect cells using baculovirusFull lengthBC049947
PAD2, ActiveP312-318BGHumanGSTSf9 insect cells using baculovirusFull lengthNM_007365
PAD3, ActiveP312-310CGHumanGSTSf9 insect cells using baculovirusFull lengthNM_016233
PAD4, ActiveP312-310DGHumanGSTSf9 insect cells using baculovirusFull lengthBC025718
PAD Cocktail, ActiveP312-37CGSTSf9 insect cells using baculovirusFull LengthNM_013358 (PAD1)
PAD2 (mouse) Inhibitor Screening KitP310BG-863
PAD1 siRNA Set IP312-911NM_013358
PAD2 siRNA Set IP312-911BBC049947
PAD3 siRNA Set IP312-911CNM_016233
PAD4 siRNA Set IP312-911DBC025718
PAD6 siRNA Set IP312-911ENM_207421


Assay Workflow:


Technical Manual:


CoA Sheet:


SDS:



There are no related publications available for this product.


RESEARCH AREAS

Inflammation


RELATED PRODUCTS


  PAD Detection Kit, P312-863

  PAD Cocktail Inhibitor Screening Kit, P37C-863

  PAD4 Inhibitor Screening Kit, P310DG-863

  PAD1 siRNA Set I, P312-911


SIGNALCHEM BIOTECH INC.


About

Terms of Service

Privacy Policy

Get the latest resources, updates and offers in your inbox