Overview:
The anti-nCoV S1 Human IgM ELISA Kit is an enzyme-linked immunosorbent assay for the detection of Human anti-nCoV Spike protein S1 IgM in serum or other biological samples. Based on indirect ELISA principles, the anti-nCoV S1 Human IgM ELISA employs recombinant nCoV Spike protein RBD coated on a 96-well plate. Controls and samples (with necessary dilution) are pipetted into the wells; the target IgM in the controls and samples binds to the immobilized Spike protein. The intensity of color development in the wells is proportional to the amount of target IgM bound.
Components:
| SignalChem Cat# | Component Name | Size | Storage |
| WB20-09 | Wash Buffer, 20X | 15 ml x 2 vials | Store kit and contents at 4oC for up to 6 months. Store unused multi well strips in the provided resealable bag and use within one month after opening. |
| C19S1M-POS | IgM Positive Control | 1 ml x 1 vial | |
| C19S1M-NEG | IgM Negative Control | 1 ml x 1 vial | |
| SS01-09 | Stop Solution | 6 ml x 1 vial | |
| CS02-506 | One-Step TMB Substrate | 10 ml x 1 vial | |
| E02-09 | nCoV Ig Sample Dilution Buffer | 10 ml x 2 vials | |
| CP03-C19SD | 2019-nCoV Spike Protein RBD Coated Plate | 1 plate | |
| H38M-63DG | Anti-Human IgM Fc Detection Antibody, 1X | 10 ml x 1 vial |
References:
1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin.
Nature. 2020, 579:270-89.
2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.
3. Long Q, et al: Antibody responses to SARS-CoV-2 in patients with COVID-19 Quan-Xin Long. Nature
Medicine. 2020, 29 April.
4. Ma H, et al: Serum IgA, IgM, and IgG responses in COVID-19. Cell Mol Immunol. 2020, 17: 773-775.
Representative Data:
Calculation and interpretation of assay results.
Related Products:
| Name | Catalog Number | Species | Tag | Expression System | Sequence | Genbank Number |
|---|---|---|---|---|---|---|
| 2019-nCoV Spike protein RBD, Fc Tag | C19SD-G241F | SARS-CoV-2 | Fc | CHO cells | 319-541 | MN908947 |
| 2019-nCoV Spike protein RBD, HIS Tag | C19SD-G241H | SARS-CoV-2 | HIS | CHO cells | 319-541 | MN908947 |
| 2019-nCoV Spike protein S1, Fc Tag | C19S1-G241F | SARS-CoV-2 | Fc | CHO cells | 16-685 | MN908947 |
| 2019-nCoV Spike protein S1, HIS Tag | C19S1-G241H | SARS-CoV-2 | HIS | CHO cells | 16-685 | MN908947 |
| 2019-nCoV Spike protein S2, Fc Tag | C19S2-G241F | SARS-CoV-2 | Fc | CHO cells | 686-1212 | MN908947 |
| Anti 2019-nCoV Spike Protein hIgG Antibody, HRP conjugated | C19S1-60DH | Human, IgG1 | CHO cells | |||
| Anti 2019-nCoV Spike Protein mIgG Antibody | C19S1-61MH | Human Fab – Mouse IgG Fc | CHO cells | |||
| Anti-Human IgM Antibody mIgG, Unconjugated | H38M-60M | Mouse, IgG | ||||
| Anti 2019-nCoV N-Protein hIgG Antibody, HRP conjugated | C19NP-60DH | Human, IgG1 | CHO cells | |||
| 2019-nCoV S1 Protein ELISA Kit | C19SD-876 | |||||
| 2019-nCoV S1 Human IgG ELISA Kit | C19S1-877 | |||||
| 2019-nCoV N-S1 Human IgM ELISA Kit | C19NS1-M877 |
Assay Workflow:
Technical Manual:
CoA Sheet:
SDS:
There are no related publications available for this product.
2019-nCoV Spike protein RBD, Fc Tag, C19SD-G241F
2019-nCoV Spike protein S1, HIS Tag, C19S1-G241H
Anti-Human IgM Antibody mIgG, Unconjugated, H38M-60M
2019-nCoV N-S1 Human IgM ELISA Kit, C19NS1-M877
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