Specificity:

Recognizes the Cyclin A protein

Cross Reactivity:

Human, Mouse and Rat

Host / Isotype / Clone#:

Rabbit, IgG

Immunogen:

Anti-Cyclin A Antibody was produced by repeated immunizations with a recombinant protein corresponding to the human cyclin A.

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Immunohistochemistry using Rabbit Anti-Cyclin A antibody. Tissue:human testes tissue. Fixation: formalin fixed paraffin embedded.Antigen retrieval: not required.Primary antibody: Anti-Cyclin A at 1:500 for 1 hr at RT. Localization: Cyclin A is nuclear and cytoplasmic.Staining: Cyclin A as precipitated red signal with hematoxylin purple nuclear counterstain.

Sample Data:

Western Blot of Rabbit Anti-Cyclin A antibody. Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr).Lane 3: T98G cells treated with PIN1 knockdown (kd1).Load: 25 µg per lane. Primary antibody: Anti-Cyclin A at 1:400 for overnight at 4°C. Predicted/Observed size: 60kDa for Cyclin A.

References :

1. Rizzolio F, Lucchetti C, et al, Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1. Cell Death and Differentiation 1-10. Nature 2012.