Specificity:

Recognizes the p21CIP1 (p21/WAF1) protein

Cross Reactivity:

Human

Host / Isotype / Clone#:

Mouse, IgG

Immunogen:

Produced by repeated immunizations with recombinant human p21 fusion protein.

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Anti-p21CIP1 (p21/WAF1) detects human p21 by western blot. 10 µg of a HeLa whole cell lysate was loaded per lane and incubated with a 1:1,000 of Anti-p21CIP1 (p21/WAF1). A single band corresponding to human p21WAF1 is detected at ~21 kDa.

Sample Data:

Immunohistochemistry of Anti-p21CIP1 (p21/WAF1) antibody 1:250. Tissue: human brain. Co-stained with YFP and Sox2 antibodies. Localization: p21 is nuclear and cytoplasmic. Staining: p21CIP1 as precipitated blue with Cy5, YFP as precipitated green with Cy2, and Sox2 as precipitated red with Cy3. z-stacks from confocal expressed as one composite focal plane.

References :

1. el-Deiry, W S. et al: WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis. Cancer Res. 1994;54(5):1169-74.

2. Wagner, M. et al: Cyclin-dependent kinase-inhibitor 1 (CDKN1A) in the squamous epithelium of the oropharynx: possible implications of molecular biology and compartmentation. Anticancer Res. 2001; 21(1A):333-45.