Rat Monoclonal Antibody
Catalog No. A17-61A
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
A17-61A-100 | 100 ug | $675 | |
A17-61A-BULK | BULK | Contact Us |
Overview:
AKT2 or Protein Kinase B β (PKBβ) is a serine/threonine kinase that is a member of the AKT family. AKT2 like the other AKT members is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility. The PI3K generates phosphatidylinositol-3,4,5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of AKT2 to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1).
References:
1. Coffer, PJ. et al: Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 1998 Oct 1; 335 ( Pt 1):1-13.
2. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.
Specificity:
Recognizes phosphorylated and unphosphorylated human AKT2 protein.
Cross Reactivity:
Human
Host Isotype / Clone#:
Rat, IgG2a
Immunogen:
The antibody was produced against synthesized peptide corresponding to internal residues of the human AKT2 protein.
Purification:
Protein G chromatography
Stability:
1yr at –20oC from date of shipment
Sample Data:
Western Blot of Anti-AKT2 (1:1,000). Lane 1: C2C12. Lane 2: MEF#1. Lane 3: MEF#2. Lane 4: A549. Lane 5: Calu-1. Lane 6: PC3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Load: 35 µg per lane.
Sample Data:
Immunohistochemical analysis of FFPE buffered formalin 10% Conc. Fixed human breast carcinoma tissue using Anti-AKT2
AS Brown et al., Identification and characterization of an hnRNP E1 translational silencing motif. Nucleir Acid Research July 2016 10.1093/nar/gkw241
L. Parker Benjamin et al., Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry Science Signaling June 2015 10.1126/scisignal.aaa3139
Zenga in et al., Lanthionine synthetase C-like protein 2 (LanCL2) is a novel regulator of Akt Molecular Biology of the Cell September 2014 10.1091/mbc.E14-01-0004
Bavelloni Alberto et al., Prohibitin 2 represents a novel nuclear AKT substrate during all-trans retinoic acid-induced differentiation of acute promyelocytic leukemia cells FASEB Journal February 2014 10.1096/fj.13-244368
Pecorari Luisa et al., Elongation Factor 1 alpha interacts with phospho-Akt in breast cancer cells and regulates their proliferation, survival and motility Molecular Cancer August 2009 10.1186/1476-4598-8-58
Treebak Jonas et al., Identification of a novel phosphorylation site on TBC1D4 regulated by AMP-activated protein kinase in skeletal muscle American Journal of Physiology - Cell Physiology November 2009 10.1152/ajpcell.00297.2009
Zhang Mingpeng et al., Poly r (C) binding protein (PCBP) 1 is a negative regulator of thyroid carcinoma AM J Transl Res August 2016
M Zhang et al., Poly r(C) binding protein (PCBP) 1 is a negative regulator of thyroid carcinoma. American Journal of Translational Research August 2016
Brown AS et al., IDENTIFICATION OF A PHOSPHO-HNRNP E1 NUCLEIC ACID CONSENSUS
SEQUENCE MEDIATING EPITHELIAL TO MESENCHYMAL TRANSITION Thesis January 2015
AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling
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