Rabbit Polyclonal Antibody
Catalog No. P57-63R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
P57-63R-100 | 100 ug | $675 | |
P57-63R-BULK | BULK | Contact Us |
Overview:
PDHK1 or Pyruvate Dehydrogenase Kinase 1 is a member of the PDHK family that phosphorylate and inactivate the Pyruvate Dehydrogenase (PDH) (1). PDH is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. PDHK1 is an important regulator of PDH in clonal β-cells and the activity of PDHK1 and PDH are important for efficient metabolic coupling. Maintaining low PDHK1 expression/activity and keeping PDH in a dephosphorylated and active state, is important for β -cells to achieve the metabolic flux rates necessary for maximal glucose-stimulated insulin secretion (2).
References:
1. Gudi, R. et al: Diversity of the pyruvate dehydrogenase kinase gene family in humans. J. Biol. Chem. 270: 28989-28994, 1995.
2. Krus, U. et al: Pyruvate dehydrogenase kinase 1 controls mitochondrial metabolism and insulin secretion in INS-1 832/13 clonal beta-cells. Biochem J. 2010 Jul 1;429(1):205-13.
Specificity:
Recognizes the human PDK-1 protein
Cross Reactivity:
Human
Host Isotype / Clone#:
Rabbit, IgG
Immunogen:
The antibody was produced against synthesized peptide corresponding to a region near the C-Terminal of human PDK-1 protein
Purification:
Immunoaffinity chromatography
Stability:
1yr at –20oC from date of shipment
Sample Data:
Western blot using affinity purified Anti-PDHK1 antibody (1:750 dilution) shows detection of myc-tagged human PDK-1 at 60kDa in ~10 µg of a virus infected Sf9 cell lysate (arrow).
There are no related publications available for this product.
Cancer, Cardiovascular Disease, Metabolic Disorder, Ser/Thr Kinases
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