Specificity:

Recognizes the BCL2 protein

Cross Reactivity:

Immunoprecipitation of BCL2 from human cells

Western blot of BCL2 from human cells.

BCL2 from other species may also be detectable.

Host:

Rabbit

Immunogen:

Synthetic peptide corresponding to amino acids 54-58 of human BCL2

Purification:

Affinity chromatography

Stability:

Store at 4°C (add 0.1% NaN₃) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Immunoprecipitation:

Representative immunoprecipitation with 2 μg of Anti-BCL2 using 500 μg of HeLa, HEK-293T, and Jurkat cell lysate.

Sample Western Blot:

Representative western blot with Anti-BCL2 (1:1000) using 20 ng of GST tagged human recombinant BCL2 (left image) or 3 μg, 10 μg, and 30 μg of Jurkat cell lysate..