Anti-AMPK alpha 1/2
Rabbit Polyclonal Antibody
Catalog No. P47-63BR
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| P47-63BR-100 | 100 ug | $270 | |
| P47-63BR-BULK | BULK | Contact Us |
Rabbit Polyclonal Antibody
Catalog No. P47-63BR
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| P47-63BR-100 | 100 ug | $270 | |
| P47-63BR-BULK | BULK | Contact Us |
Overview:
AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis (1). AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) (2) and via signaling pathways in response to adiponectin, leptin and CAMKKβ.
References:
1. Hardie, G.D. The AMP-activated protein kinase pathway – new players upstream and downstream. J. Cell Sci. 2004;117: 5479–5487.
2. Kahn, B.B. et al. AMP-activated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab; 2005: 1, 15–25.
Specificity:
Recognizes the AMPK1 and AMPK2 protein
Cross Reactivity:
Western blot human AMPK1 and AMPK2
AMPK1 and AMPK2 from other species may also be detectable.
Host:
Rabbit
Immunogen:
Synthetic peptide corresponding to amino acids 170-174 of human AMPK
Purification:
Affinity chromatography
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Representative western blot with Anti-AMPK1/2 (1:1000) using 20 ng of human recombinant AMPK1 and AMPK2.
WBgel.jpg)
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N Aznar et al., AMP-activated protein kinase fortifies epithelial tight junctions during energetic stress via its effector GIV/Girdin. Elife November 2016 10.7554/eLife.20795
PM Anders et al., The KSHV K1 Protein Modulates AMPK Function to Enhance Cell Survival. PLoS Pathogens November 2016 10.1371/journal.ppat.1005985
W. Zmijewski Jaroslaw et al., Exposure to Hydrogen Peroxide Induces Oxidation and Activation of AMP-activated Protein Kinase Journal of Biological Chemistry October 2010 10.1074/jbc.M110.143685
M. Beauchamp Elspeth et al., Direct Binding of Arsenic Trioxide to AMPK and Generation of Inhibitory Effects on Acute Myeloid Leukemia Precursors Molecular Cancer Therapeutics October 2014 10.1158/1535-7163.MCT-14-0665-T
Frøsig Christian et al., Exercise-induced TBC1D1 Ser237 phosphorylation and 14-3-3 protein binding capacity in human skeletal muscle Journal of Physiology September 2010 10.1113/jphysiol.2010.194811
JD Klein et al., Metformin, an AMPK activator, stimulates the phosphorylation of aquaporin 2 and urea transporter A1 in inner medullary collecting ducts. American Journal of Physiology - Renal Physiology May 2016 10.1152/ajprenal.00102.2016
B Eric et al., Discovery of TBC1D1 as an Insulin-, AICAR-, and Contraction-stimulated Signaling Nexus in Mouse Skeletal Muscle Journal of Biological Chemistry April 2008 10.1074/jbc.M708839200
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