Rabbit Polyclonal Antibody
Catalog No. P51-63R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
P51-63R-25 | 25 ug | $115 | |
P51-63R-100 | 100 ug | $395 | |
P51-63R-BULK | BULK | Contact Us |
Overview:
The second messenger cyclic AMP (cAMP) activates cAMP-dependent protein kinase (PKA or cAPK) in mammalian cells and controls many cellular mechanismssuch as gene transcription, ion transport and protein phosphorylation (1). Inactive PKA is a heterotetramer composed of a regulatory subunit (R) dimer and a catalytic subunit (C) dimer, and in this inactive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits. Three C subunit isoforms (-cα, -cβand -cγ) and two families of regulatory subunits (RI and RII) with distinct cAMP binding properties have been identified. Upon binding of cAMP to the R subunits, the autoinhibitory contact is eased and active monomeric C subunits are released.
References:
1. Montminy M: Transcriptional regulation by cyclic AMP. Annu Rev Biochem, 1997;66:807-22.
Specificity:
Recognizes the PKAcα, PKAcβ and PKAcγ protein
Cross Reactivity:
Western blot human PKAca, PKAcB and PKAcy
PKAca, PKAcB and PKAcy from other species may also be detectable.
Host:
Rabbit
Immunogen:
Protein Code: P17612
Purification:
Affinity purified using Protein G
Stability:
Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Western Blot:
Representative western blot with Anti-PKAc (1:250) using 20ng of PKAcα, PKAcβ and PKAcγ recombinant protein.
A Thorne Curtis et al., Small-molecule inhibition of Wnt signaling through activation of casein kinase 1α Nature Chemical Biology November 2010 10.1038/nchembio.453
Yamaguchi Fuminori et al., S100 Proteins Modulate Protein Phosphatase 5 FunctionA LINK BETWEEN CA2+ SIGNAL TRANSDUCTION AND PROTEIN DEPHOSPHORYLATION Journal of Biological Chemistry April 2012 10.1074/jbc.M111.329771
S Shah et al., Regulation of FcRγ function by site-specific serine phosphorylation. Journal of Leukocyte Biology September 2016 10.1189/jlb.2AB0516-228R
J. Coultrap Steven et al., Improving a Natural CaMKII Inhibitor by Random and Rational Design PLoS One October 2011 10.1371/journal.pone.0025245
Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112
Apoptosis/Autophagy, Cardiovascular Disease, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, PKA/PKC Pathway
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