Rabbit Polyclonal Antibody
Catalog No. E10-63BR
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
E10-63BR-100 | 100 ug | $270 | |
E10-63BR-BULK | BULK | Contact Us |
Overview:
EEGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility, and cell survival (1). Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms (2). Activation of EGFR triggers activation of the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation.
References:
1. Wang K, et al: Epidermal growth factor receptor-deficient mice
have delayed primary endochondral ossification because of
defective osteoclast recruitment. J. Biol. Chem. 279: 53848-53856,
2004.
2. Kobayashi S, et al: EGFR mutation and resistance of non-small-cell
lung cancer to gefitinib. New Eng. J. Med. 352: 786-792, 2005.
Specificity:
Recognizes the EGFR protein
Cross Reactivity:
Western blot EGFR from human cells
EGFR from other species may also be detectable.
Host :
Rabbit
Immunogen:
Synthetic peptide corresponding to amino acids 1195- 1199 of human EGFR (protein accession number P00533)
Purification:
Affinity chromatography
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Representative western blot with Anti-EGFR (1:1000) using 3 μg, 10 μg and 30 μg of HeLa cell lysate.
DE Heppner et al., The NADPH Oxidases DUOX1 and NOX2 Play Distinct Roles in Redox Regulation of Epidermal Growth Factor Receptor Signaling. Journal of Biological Chemistry October 2016 10.1074/jbc.M116.749028
Lia Feng et al., Screening of epidermal growth factor receptor inhibitors in natural products by capillary electrophoresis combined with high performance liquid chromatography-tandem mass spectrometry Journal of Chromatography A June 2015 10.1016/j.chroma.2015.04.055
Rao Suman et al., Target Modulation by a Kinase Inhibitor Engineered to Induce a Tandem Blockade of the Epidermal Growth Factor Receptor (EGFR) and c-Src: The Concept of Type III Combi-Targeting PLoS One February 2015 10.1371/journal.pone.0117215
Krejci Pavel et al., Receptor Tyrosine Kinases Activate Canonical WNT/β-Catenin Signaling via MAP Kinase/LRP6 Pathway and Direct β-Catenin Phosphorylation PLoS One April 2012 10.1371/journal.pone.0035826
AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, PKA/PKC Pathway, Receptor Tyrosine Kinases
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