Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
B10-63R-100	100 ug	$595
B10-63R-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

B10-63R-100

100 ug

$595

B10-63R-BULK

BULK

Overview:

BTK (also known as Bruton tyrosine kinase) plays a crucial role in B-lymphocyte differentiation and activation. BTK interacts with SRC homology 3 domains of FYN, LYN and HCK that are activated upon stimulation of B- and T-cell receptors (1). Defects in the BTK gene cause Agammaglobulinemia, an X-linked immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. The unique role of BTK makes it a desirable target for potential anti-cancer, anti-inflammatory and anti-viral agents as well as other treatments (2).

References:

1. Cheng, G. et al: Binding of Bruton's tyrosine kinase to Fyn, Lyn, or Hck through a Src homology 3 domain-mediated interaction. Proc. Nat. Acad. Sci. 91: 8152-8155, 1994.

2. Vassilev, A O. et al: Therapeutic potential of inhibiting Bruton's tyrosine kinase, (BTK). Curr Pharm Des. 2004;10(15):1757-66.

Specificity:

Recognizes the BTK protein

Cross Reactivity:

Human and Mouse

Host / Isotype / Clone#:

Rabbit, IgG

Immunogen:

BTK antibody was raised against a synthetic peptide corresponding to 16 amino acids near the amino-terminus of human BTK

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Western blot analysis of BTK in U937 cell lysate with BTK antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.