Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
M13-63BR-100	100 ug	$595
M13-63BR-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

M13-63BR-100

100 ug

$595

M13-63BR-BULK

BULK

Overview:

ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Overexpression of ASK1 induces apoptotic cell death, and ASK1 is activated in cells treated with tumor necrosis factor-alpha (1). ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation (2).

References:

1. Smith, C. Ichijo, H. et al: Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 275: 90-94, 1997.

2. Nishitoh, H. et al: ASK1 is essential for JNK/SAPK activation by TRAF2. Molec. Cell 2: 389-395, 1998.

Specificity:

Recognizes the ASK1 protein

Cross Reactivity:

Human and Mouse

Host:

Rabbit, IgG

Immunogen:

ASK1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ASK1.

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Western blot analysis of ASK1 in SW1353 cell lysate with ASK1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.