Rabbit Polyclonal Antibody
Catalog No. T02-63R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
T02-63R-100 | 100 ug | $595 | |
T02-63R-BULK | BULK | Contact Us |
Overview:
TBK1, also known as NAK or NFkB-activating kinase, is an upstream protein kinase that can phosphorylate and activate the IkB kinases (1). Activation of IkB kinases allows the phosphorylation of IkB protein which is then degraded via the ubiquitination pathway. This mechanism allows the activation of the NFkB transcriptional complex. TBK1 is a specific upstream regulator of IkB kinases and can also interact and the IkB protein TANK. TBK1 is a component of the virus-activated kinase that phosphorylate IRF3 and IRF7 allowing their dimerization and translocation to the nucleus, where they induce transcription of interferon (2).
References:
1. Tojima, Y. et al: NAK is an I-kappa-B kinase-activating kinase. Nature 404: 778-782, 2000.
2. Sharma, S. et al: Triggering the interferon antiviral response through an IKK-related pathway. Science 300: 1148-1151, 2003.
Specificity:
Recognizes the TBK1 protein
Cross Reactivity:
Human and Mouse
Host / Isotype / Clone#:
Rabbit, IgG
Immunogen:
TBK1 antibody was raised against a synthetic peptide corresponding to 17 amino acids form near the carboxy terminus of human TBK1.
Purification:
Affinity chromatography
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Immunocytochemistry of TBK1 in MOLT4 cells with NAK antibody at 10 ug/ml.
Hong Park Sun et al., IRAK4 as a Molecular Target in the Amelioration of Innate Immunity?Related Endotoxic Shock and Acute Liver Injury by Chlorogenic Acid Journal of Immunology November 2014 10.4049/jimmunol.1402101
Young Leea Jin et al., Celastrol blocks binding of lipopolysaccharides to a Toll-like receptor4/myeloid differentiation factor2 complex in a thiol-dependent manner Journal of Ethnopharmacology August 2015 10.1016/j.jep.2015.06.028
Young Lee Jin et al., Gambogic Acid Disrupts Toll-like Receptor4 Activation by Blocking Lipopolysaccharides Binding to Myeloid Differentiation Factor 2 Toxicological Research March 2015 10.5487/TR.2015.31.1.011
Cytoplasmic Tyrosine Kinases, Inflammation, NfkB Pathway
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