Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
X08-63R-100	100 ug	$595
X08-63R-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

X08-63R-100

100 ug

$595

X08-63R-BULK

BULK

Overview:

XIAP is a member of a family of proteins which inhibit apoptosis and act as mammalian cell-death suppressors. XIAP inhibits at least 2 members of the caspase family of cell-death proteases, caspase-3 and caspase-7 (1). In addition, XIAP inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. Furthermore, XIAP can mediate protection of cells from apoptosis by utilizing both a JNK1 activation pathway that involves ILPIP and a caspase inhibition pathway that is independent of ILPIP (2). Disruption of the XIAP gene in human colon cancer cells can enhance sensitivity of these cells to exogenously added TRAIL suggesting that XIAP is a nonredundant modulator of TRAIL-mediated apoptosis.

References:

1. Deveraux Q L, et al: X-linked X-linked IAP is a direct inhibitor of cell-death proteases. Nature 388: 300-304, 1997.

2. Tang G, et al: Inhibition of JNK activation through NF- kappa-B target genes. Nature 414: 313-317, 2001.

Specificity:

Recognizes the XIAP protein

Cross Reactivity:

Human and Mouse

Host / Isotype / Clone#:

Rabbit, IgG

Immunogen:

XIAP antibody was raised against a synthetic peptide corresponding to 13 amino acids at the C-terminus of human XIAP

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Western blot analysis of XIAP in human kidney lysate with XIAP antibody at 0.5 (lane A), 1 (lane B), and 2 (lane C) ug/ml, respectively.