Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
J02-63R-100	100 ug	$315
J02-63R-BULK	BULK	Contact Us

Overview:

JAK2 is a member of intracellular non-receptor tyrosine kinases that transduce cytokine-mediated signals via the JAK-STAT pathway (1). JAK2 has two near-identical phosphate-transferring domains. One domain exhibits the kinase activity while the other stabilizes the JAK conformational structure. JAK2 is the predominant JAK kinase activated in response to several growth factors and cytokines such as IL-3, GM-CSF and erythropoietin. JAK2 has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon (2).

References:

1. Lacronique, V. Et al : A TEL-JAK2 fusion protein with constitutive kinase activity in human leukemia. Science, 1997; 278: 1309-1312.

2. Ho, J.M. et al: TEL-JAK2 constitutively activates the extracellular signal-regulated kinase (ERK), stress-activated protein/Jun kinase (SAPK/JNK), and p38 signaling pathways. Blood, 2002; 100: 1438-1448.

Specificity:

Recognizes the JAK2 protein

Cross Reactivity:

Western blot JAK2 from human and mouse cells

JAK2 from other species may also be detectable.

Host:

Rabbit

Immunogen:

Synthetic peptide corresponding to amino acids 1005-1009 of human JAK2

Purification:

Affinity chromatography

Stability:

Store at 4°C (add 0.1% NaN₃) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Representative western blot with Anti-JAK2 (1:1000) using 20 ng of human recombinant JAK1 and JAK2.