Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
C48-60M-25	25 ug	$110
C48-60M-100	100 ug	$395
C48-60M-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

C48-60M-25

25 ug

$110

C48-60M-100

100 ug

$395

C48-60M-BULK

BULK

Overview:

CHK2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage; the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner (1). Expression of wild-type Chk2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative Chk2 mutant abrogated both phosphorylation of p53 on Ser-20 and p53 stabilization (2).

References:

1. Matsuoka, S. et al: Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998 Dec 4;282(5395):1893-7.

2. Chehab NH. et al: Chk2/hCds1 functions as a DNA damage checkpoint in G(1) by stabilizing p53. Genes Dev. 2000 Feb 1;14(3):278-88.

Specificity:

Recognizes the CHK2 protein

Cross Reactivity:

Western blot CHK2 from human and mouse cells

CHK2 from other species may also be detectable.

Host / Isotype / Clone#:

Mouse, IgG1, 7F9

Immunogen:

Human full length recombinant CHK1

Purification:

Affinity Chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Representative western blot (1:1000) with Anti-CHK2 using 20 ng of GST-tagged human full length recombinant CHK1 and CHK2.