Home   >    Reagents   >   siRNAs

PAD2 siRNA Set I

PAD2 is a pool of three individual synthetic siRNA duplexes designed to knock-down human PAD2 mRNA expression. Each siRNA is 19-25 bases in length.
Catalog No. P312-911B


For enquiry on all siRNA Sets email us at orders@signalchem.com


Catalog No. Pack Size Price (USD)
P312-911B-05 5 nmol $655
P312-911B-20 20 nmol $1205
P312-911B-50 50 nmol $2085
P312-911B-BULK BULK Contact Us  


Overview:

PAD2 is a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. PAD2 has peptidylarginine deiminase activity against synthetic substrates (1). PAD2 is mainly expressed in the central nervous system, skeletal muscle, spinal cord, cerebrum, cerebellum, and submaxillary gland (2). PAD2 play a role in the onset and progression of neurodegenerative human disorders, including Alzheimer disease and multiple sclerosis, and it has also been implicated in glaucoma pathogenesis.


Gene Aliases:

PADI2; mKIAA0994


Genbank Number:


References:


1. Ishigami, A. et.al: Human peptidylarginine deiminase type II: molecular cloning, gene organization, and expression in human skin. Arch. Biochem. Biophys. 407: 25-31, 2002.

2. Watanabe, K. et.al: Isolation and characterization of cDNA clones encoding rat skeletal muscle peptidylarginine deiminase. J. Biol. Chem. 264: 15255-15260, 1989.


Specificity:

PAD2 siRNAs are designed to specifically knock-down human PAD2 expression.


Reconstitution Protocol:

Each siRNA duplex is supplied as 2.5nmol per tube (for example 6 x 2.5nmol = 3 x 5nmol per duplex). Briefly centrifuge one tube per duplex (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend each 2.5nmol tube in 50 ul of DEPC-treated water (included in kit), which results in a 1x stock solution (50 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.


Storage and Stability:

The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Format:

Lyophilized powder



Product Datasheets



There are no related publications available for this product.


RESEARCH AREAS

Inflammation


RELATED PRODUCTS


  PAD2 (human) Inhibitor Screening Kit, P318BG-863

  PAD1 Inhibitor Screening Kit, P310G-863

  PAD4 Inhibitor Screening Kit, P310DG-863

  PAD3 Inhibitor Screening Kit, P310CG-863


SIGNALCHEM BIOTECH INC.


About

Terms of Service

Privacy Policy

Get the latest resources, updates and offers in your inbox